The Wilmot Proviso aimed to prohibit slavery in territories acquired from which country?

The Wilmot Proviso specifically aimed to prohibit slavery in territories that were acquired from Mexico as a result of the Mexican-American War (1846-1848). This proposal arose in the context of intense debates over the expansion of slavery into new territories, reflecting the growing tensions between pro-slavery and anti-slavery factions in the United States during the mid-19th century.

The significance of the Wilmot Proviso lies in its challenge to the status quo regarding slavery, and it attempted to address the issue before any new states or territories were formed from the lands acquired through the conflict with Mexico. This legislative effort represented a pivotal moment in pre-Civil War America, emphasizing the contentious nature of slavery and its extension, which ultimately played a crucial role in the country's historical trajectory.

The other countries listed in the options—Canada, Spain, and France—are not relevant to the context of the Wilmot Proviso, as the Proviso was not concerned with territories acquired from them.